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  • Rapid detection of E. coli cells in urine samples using a ...

    Escherichia coli is one of the main causes of urinary tract infections (UTIs). E. coli is commonly detected from urine using standard culture method. However, the urine sampling and analysis required for these methods can be costly, time consuming (requires 24 to 48 hours) and labor-intensive. This …

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  • Separating and detecting escherichia coli in a ...

    2011-8-1 · We report a lab-on-a-chip (LOC) that can separate and detect Escherichia coli (E. coli) in simulated urine samples for urinary tract infection (UTI) applications. The LOC consists of two (concentration and sensing) chambers connected in series and an integrated impedance detector.

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  • Quantitative detection of Escherichia coli from urine

    2012-12-1 · Methods: The gene encoding the universal stress protein (uspA) was found to be highly specific for E. coli. We quantified the copy numbers of E. coli in the urine of patients with UTI by using a real-time PCR assay (the TaqMan® system) targeting uspA genes in genomic DNAs isolated from urine samples (n = 200). To evaluate the feasibility of this method, the results were compared with those of …

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  • A multiplex PCR method to detect 14 Escherichia coli ...

    E. coli strains belonging to 14 serogroups, including O1, O2, O4, O6, O7, O8, O15, O16, O18, O21, O22, O25, O75 and O83, are the most frequently detected UPEC strains in a diverse range of clinical urine specimens. In the current study, the O-antigen gene clusters of E. coli serogroups O1, O2, O18 and O75 were characterized.

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  • Application of a Micro-Machined Electronic Nose to

    2019-6-18 · Soprani, M.; Zambotti, G.; Gobbi, E.; Ponzoni, A. Application of a Micro-Machined Electronic Nose to Detect Escherichia Coli in Human Urine Samples. Proceedings 2019, 14, 3. https://doi.org/10.3390/proceedings2019014003

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  • Urine Culture with E. Coli: Meaning, Results and

    2021-6-23 · The presence of E. coli in urine usually indicates a urinary tract infection, but it may not always cause visible symptoms. When symptoms appear, they may include a burning sensation while urinating, a strong urge to urinate, passing small amounts of urine, passing strong-smelling urine, and urine that appears cloudy or red.

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  • Sensitive and rapid detection of pathogenic bacteria

    In this study, bacteria in urine samples were enriched using a portable filter-based pipette. Then, a centrifugal chip was constructed to detect E. coli, S. aureus, S. typhimurium, P. mirabilis, and P. aeruginosa from urine samples by integrating the DNA extraction, multiplex recombinase polymerase amplification and fluorescent detection together.

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  • Microfluidic smartphone quantitation of Escherichia

    2019-12-1 · In this study we present a sensitive and rapid optical sandwich fluorescent immunoassay able to detect and quantify E. coli K12 in less than 25 min in synthetic urine without the need of sample preparation, with the assistance of a smartphone camera. The new sandwich immunossay presents several advantages, such as: 1) affordable off-the-shelf immunoassay reagents, 2) rapid procedure …

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  • E. Coli and UTIs (Urinary Tract Infections): The

    2021-6-24 · After confirming that the e-RCD assay could sensitively detect E. coli in both buffer and urine, we assessed the specificity of this assay against a panel of Gram-negative and …

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  • Quantitative detection of Escherichia coli from urine

    2012-12-1 · Quantification of E. Coli in Urine Specimens and Comparison of Results. We quantified the copy numbers of E. coli from urine specimens using the standard curve described above. In all the urine samples in which E. coli was detected, we detected 5.9 × 10 7 to 1.2 × 10 2 copies/mL of E. coli.

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  • Selective detection of Escherichia coli caused UTIs

    2019-11-1 · The aim of the present study was developing a surface plasmon resonance (SPR) nanosensor to detect Escherichia coli (E. coli) for the diagnosis of urinary tract infections by using surface imprinted Au nanoparticles (AuNPs) as a recognition element.In order to realize imprinting, Cu(II) ions were used to provide interaction between E. coli cell wall and amine functionalized AuNPs forming ...

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  • Urine Culture with E. Coli: Meaning, Results and

    2021-6-23 · The presence of E. coli in urine usually indicates a urinary tract infection, but it may not always cause visible symptoms. When symptoms appear, they may include a burning sensation while urinating, a strong urge to urinate, passing small amounts of urine, passing strong-smelling urine, and urine that appears cloudy or red.

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  • Isolation of ESBL producing E. coli from Urine •

    2013-4-18 · Microbiologic methods for detection of ESBL E.coli a. General consideration Escherichia coli is the predominant cause of both community and nosocomial urinary tract infection (UTI) and also is the commonest cause of uncomplicated UTI in women and children. Extended-spectrum ß-lactamases (ESBL) are a rapidly evolving group of ß-lactamases that share the ability to hydrolyze third-generation ...

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  • Culture-free bacterial detection and identification

    2021-4-27 · Fast detection of E. coli with a novel fluorescent biosensor based on a FRET system between UCNPs and [email protected] in urine Analytical Methods ( IF 2.596) Pub Date : 2021-4-6, DOI: Yuan Yao, Guoming Xie, Xin Zhang, Jinshan Yuan, Yulei Hou, Hui Chen

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  • Microfluidic smartphone quantitation of Escherichia

    2018-2-21 · The application of V G = 0.6 V ... blood and using the detection electrodes prepared to detect E. coli to monitor the 2-hour ... Antimicrobial Susceptibility Testing in Urine.

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  • Rapid detection of bacteria based on homogenous ...

    2019-12-1 · For full response curves, a serial dilution of E. coli K12 range from 10 8 to 10 0 CFU/mL in 3% w/w BSA or synthetic urine were loaded into a Nunc maxisorp microplate 400 μL well and 3% w/w BSA used as negative control. The MCF strip was then moved to a new well loaded with 40 μg/mL biotinylated polyclonal detection antibody (detAb) and ...

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  • E Coli in Urine Culture: Meaning & Home Treatment

    2016-10-5 · The calibration curve of E. coli with different initial concentrations (between 100 cfu mL −1 and 1 × 10 8 cfu mL −1 of E. coli). To confirm the accuracy of the fluorescence immunoassay, recovery experiments were also carried out in urine sample matrix.

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  • Sensitive and rapid detection of pathogenic bacteria

    Bacterial infections may cause severe diseases such as tuberculosis, sepsis, nephritis and cystitis. The rapid and sensitive detection of bacteria is a prerequisite for the treatment of these diseases. The current gold standard for bacterial identification is bacteriological culture. However, culture-based i Coronavirus articles - free to access collection

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  • Isolation of ESBL producing E. coli from Urine •

    2013-4-18 · Microbiologic methods for detection of ESBL E.coli a. General consideration Escherichia coli is the predominant cause of both community and nosocomial urinary tract infection (UTI) and also is the commonest cause of uncomplicated UTI in women and children. Extended-spectrum ß-lactamases (ESBL) are a rapidly evolving group of ß-lactamases that share the ability to hydrolyze third-generation ...

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  • Fast detection of E. coli with a novel fluorescent ...

    Biosensors based on nanomaterials are becoming a research hotspot for the rapid detection of pathogenic bacteria. Herein, a “turn-on” fluorescent biosensor based on a FRET system was constructed for the fast detection of a representative pathogenic microorganism, namely, E. coli, which causes most urinary tract infections.This biosensor was constructed by utilizing synthesized UCNPs as ...

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  • First Instant Test to Detect Presence of E. Coli 1057:H7

    One approach to the accurate determination of the pathogenic potential (pathotype) of isolated Escherichia coli strains would be through a complete assessment of each strain for the presence of all known E. coli virulence factors. To accomplish this, an E. coli virulence factor DNA microarray composed of 105 DNA PCR amplicons printed on glass slides and arranged in eight subarrays ...

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  • Rapid Identification of Escherichia coli Pathotypes by ...

    2018-11-5 · Electrical engineers have adapted a molecular electronic device called a single-molecule break junction to detect RNA from strains of E. coli known for causing illness.

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  • Detecting E. coli strains using molecular electronics ...

    New paper strips detect Escherichia coli simply, cheaply and quickly. 2 2. The E. coli-detecting paper strips developed at McMaster University (Photo: JD Howell) Nothing can put a dampener on a ...

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  • New paper strip test detects E. coli quickly, cheaply

    2007-7-30 · In a large study using 9247 urine samples, the authors reported that 94% of E. coli strains could be identified by their coloured appearance alone and no E. coli strains were misidentified. When compared with the use of conventional media (Blood and MacConkey agars), the authors reported a cost reduction of 46% for media, and a 67% reduction in ...

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  • Women with symptoms of a urinary tract infection but

    2015-3-17 · Addition of thiourea improved the typeability of Dnd + strains by 100%. Without the addition of thiourea to the PFGE run, all 12 Dnd + E. coli strains from previous studies [13,14] yielded degraded DNA despite repeated attempts (3 times) (Figure 1(a), lanes S1-B2). However, when thiourea was added to the running buffer, there was a 100% marked improvement in typeability for all the E. coli ...

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  • Escherichia coli Global Gene Expression in Urine from ...

    2010-11-11 · Of these ten E. coli-positive samples, two urine specimens contained mixed infections of two different E. coli strains. O and H serotyping conducted on these 12 E. coli isolates indicated that O6 and O25, which are frequently associated with UTI isolates [37] , were the most common serogroups, representing 7 of 12 strains ( Table S1 ).

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  • First Instant Test to Detect Presence of E. Coli 1057:H7

    2019-7-12 · Escherichia coli is the primary cause of urinary tract infection (UTI), which is one of the most frequent human infections. While much is understood about the virulence factors utilized by uropathogenic E. coli (UPEC), less is known about the bacterial growth dynamics taking place during infection. Bacterial growth is considered essential for successful host colonization and infection, and ...

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  • Growth Rate of Escherichia coli During Human Urinary

    Laboratory Diagnosis. Laboratory diagnosis of E. coli infections is based on. a) Isolation of E. coli by culture. b) Demonstration of toxins of diarrheagenic E. coli. Specimens Urine is the specimen of choice for diagnosis of UTI caused by uropathogenic E. coli.Clean-voided midstream samples of urine are usually employed for culture.

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  • Laboratory Diagnosis of Escherichia coli Infections

    2016-1-27 · Unlike approximately 92% or E. coli, E. coli O157:H7 and nonmotile E. coli O157 strains that produce Shiga-like toxins lack the enzyme and are MUG negative. For this reason the MUG assay used in conjunction with testing for sorbitol fermentation and agglutination in E. coli O 157 antiserum is a useful screening test for toxigenic strains of O 157.

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  • E. coli O157:H7: Procedure for Isolation and ...

    2021-6-25 · MicroSnap™ E. coli is a rapid test for detection and enumeration of E. coli bacteria.The test uses a novel bioluminogenic test reaction that generates light when enzymes that are characteristic of E. coli bacteria react with specialized substrates. The light-generating signal is then quantified in the EnSURE™ Touch luminometer.

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  • MicroSnap E. coli - Indicator Organisms - Hygiena

    2018-3-12 · E. coli is a member of the bacterial family of Enterobacteriaceae [].It is a most common intestinal microorganism of humans and warm-blooded animals [].Some strains of this bacterium have the pathogenic potential and can cause a wide range of infections from mild to life-threatening conditions, such as traveler’s diarrhea, infant diarrhea, hemorrhagic colitis, hemolytic uremic syndrome ...

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  • Detection Methods for Lipopolysaccharides: Past and ...

    2007-8-31 · E. Group B streptococcus should be treated with penicillin. 5. The following statements are true regarding urinary tract infection in pregnancy: A. E. coli is the commonest organism isolated from urine culture. B. Group B streptococcal vaginal colonization is associated with preterm delivery.

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  • Urinary tract infection: detection of Escherichia coli ...

    The hyperimmune serum reacted with all urine specimens studied. However, bands of 77, 66, 51, 43, and 21 kDa were detected in urine specimens positive for E. coli. The urine specimens that were positive for heterologous bacteria showed one to four bands, which were combination of …

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  • Fast detection of E. coli with a novel fluorescent ...

    Biosensors based on nanomaterials are becoming a research hotspot for the rapid detection of pathogenic bacteria. Herein, a “turn-on” fluorescent biosensor based on a FRET system was constructed for the fast detection of a representative pathogenic microorganism, namely, E. coli, which causes most urinary tract infections.This biosensor was constructed by utilizing synthesized UCNPs as ...

    Get Price
  • E Coli in Urine - Causes, Symptoms, Treatment

    2015-6-3 · E. Coli may be detected in urine during routine checkups or after a patient shows symptoms of the infections. UTIs occur in different forms with varying symptoms. Acute pyelonephritis is a urinary tract infection that affects the kidneys. A urinary tract infection of the bladder may be referred to as cystitis.

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  • First Instant Test to Detect Presence of E. Coli 1057:H7

    2020-10-10 · Diagnosis. To diagnose illness caused by E. coli infection, your doctor sends a sample of your stool to a laboratory to test for the presence of E. coli bacteria. The bacteria may be cultured to confirm the diagnosis and identify specific toxins, such as those produced by E. coli O157:H7.

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  • E. coli - Diagnosis and treatment - Mayo Clinic

    2019-5-3 · APPLICATION NOTE TaqMan Assays for urinary tract microbiota ... UTIs are diagnosed by clinical symptoms and urine analysis ... Ba04646242_s1_E. coli Ba04932083_s1_K. pneumoniae Ba04932081_s1_. aeruginosa Ba04932076_s1_abilis Ba04646247_s1_E. faecalis Ba04932086_s1_E. faecium

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  • High-throughput profiling of urinary tract microbiota ...

    2018-10-26 · Researchers in infection biology have developed a new way to detect hidden urinary tract infections. The research was led by Professor Agneta Richter-Dahlfors, head of the Swedish Medical ...

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  • A new way to detect hidden urinary tract infections

    2021-3-19 · Label one half B. subtilis and the other half E. coli. 2. Make a single streak line with the appropriate organism on the corresponding half of the plate as shown in Fig. 6. 3. Incubate upside down and stacked in the petri plate holder on the shelf of the 37°C incubator corresponding to your lab section until the next lab period. 4.

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  • Lab 8: Using Biochemical Testing to Identify Bacteria ...

    2015-6-15 · The specific binding of the mannose units to E. coli allowed quantitative detection of the bacteria at levels down to 450 colony forming units mL −1 in lab samples, and facilitate the application of the Man-CQDs for bacterial analyses of real samples (tap water, apple juice, human urine). The synthesis of our Man-CQDs, their labeling, and ...

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  • One-step synthesis of biofunctional carbon quantum

    2017-4-17 · could readily detect mock ZIKV virus at concentrations as low as 20 aM (Fig. 2D). SHERLOCK is also able to detect ZIKV in clinical isolates (serum, urine, or saliva) where titers can be as low as 2 × 10. 3. copies/mL (3.2 ) (21aM). ZIKV RNA extracted from patient serum or urine samples and reverse transcribed into cDNA

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