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  • E. coli O157:H7 and HUS Fact Sheet - Minnesota Dept.

    2017-9-6 · We have developed a new litmus paper test (DipTest) for detecting Escherichia coli (E. coli) in water samples by performing enzymatic reactions directly on the porous paper substrate. The paper strip consists of a long narrow piece of cellulose blotting paper coated with chemoattractant (at bottom edge), wax hydrophobic barrier (at the top edge), ...

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  • DipTest: A litmus test for E. coli detection in water

    2019-12-1 · Yang et al. (2011) reported an LoD of 3.4 × 10 4 CFU/mL of E. coli in synthetic urine with total assay taking 100 min. Safavieh et al. (2012) developed a microfluidic loop-mediated isothermal amplification (LAMP) with electrochemical detection presenting an LoD of 48 CFU/mL of E. coli involving sample preparation by filtration of urine and total assay time of 60 min.

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  • Microfluidic smartphone quantitation of Escherichia

    2018-11-5 · However, current methods for detecting illness-causing strains of bacteria such as E. coli require either time-intensive biological cell cultures or DNA amplification approaches that rely on ...

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  • Detecting E. coli strains using molecular electronics ...

    2020-2-1 · Our novel method was able to detect E. coli of phylogenetic groups A, B1, B2, D, F, and clade V across diverse sample types, including a vast majority of uropathogenic E. coli. Moreover, the novel (Nextgen) PCR thermal cycler allowed ultrafast PCR amplification, in 16 min (total E. coli identification in 52 min, including DNA extraction, PCR, electrophoresis, staining, destaining and visualization under UV).

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  • Fast identification of Escherichia coli in urinary tract ...

    Taking into account the presence of yehXYWZ in all four sequenced E. coli genomes, the encoded proteins showed more than 40% sequence identity over more than 70% of the query sequence length (ABC subunit OpuCA), more than 40% sequence identity over more than 80% of the query sequence length (integral membrane protein subunits OpuCB and OpuCD) or more than 25% sequence identity over 97% of the alignment length …

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  • Osmoregulatory Systems of Escherichia

    We could detect 10 2 CFU mL −1 of E. coli from urine samples within 15 min. The time to positivity (Tp) for each bacterium was measured from the time at which the fluorescence exceeds a threshold value equal to three times the standard deviation of the negative controls (urine with no bacteria).

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  • Sensitive and rapid detection of pathogenic bacteria

    2018-5-18 · Patients with a previous antimicrobial consumption were excluded from the study. Isolation and identification of E. coli was performed by a standard procedure. Briefly, blood and urine samples were cultured onto Eosin Methylene Blue agar (Merck, Germany) and MacConkey agar (Merck, Germany) at 37 °C for 24 h.

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  • Imipenem resistance in clinical Escherichia coli from

    2014-11-13 · We first demonstrate that, in buffer, the DNAzyme sensor system is able to detect single-target E. coli K12 that is lysed in a droplet (5 μm in diameter) within 8 min (Supplementary Fig. 4). This ...

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  • Separating and Detecting Escherichia Coli in a ...

    2011-8-12 · detect Escherichia Coli (E. coli) in simulated urine samples for Urinary Tract Infection (UTI) diagnosis. The LOC consists of two (concentration and sensing) chambers connected in series and an integrated impedance detector. The two-chamber approach is designed to reduce the non-

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  • Assessment of technique for rapid detection of

    E. coli or P. mirabilis mixed with significant numbers of another organism were not detected in four out of five urines. The technique is simple and could be automated. It appears to merit more extensive trial in a hospital laboratory and further development to detect and correctly identify more species that cause urinary tract infections.

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  • E. coli in Urine - Causes, Symptoms, Treatment

    Urine culture is the gold standard for diagnosing UTI. Standard urine culture techniques aim to detect a defined set of uropathogens, such as Escherichia coli, that commonly cause UTI. However, a substantial number of urine cultures from symptomatic patients yield negative results.

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  • Moving Beyond E coli: New Techniques, Technologies ...

    2007-12-18 · An E. coli isolate from a cystitis patient with findings of urine IBCs and filamentous bacteria was inoculated by transurethral catheterization into the bladders of 8-wk-old female C3H/HeN mice (Harlan-Sprague Dawley) as previously described in order to compare human and mouse urine cytology. Mouse urine samples were collected 30 h ...

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  • Detection of Intracellular Bacterial Communities in

    2021-4-27 · Fast detection of E. coli with a novel fluorescent biosensor based on a FRET system between UCNPs and [email protected] in urine Analytical Methods ( IF 2.596) Pub Date : 2021-4-6, DOI: Yuan Yao, Guoming Xie, Xin Zhang, Jinshan Yuan, Yulei Hou, Hui Chen

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  • Mutant Bacteria Will Test You For Disease And Color

    2015-5-27 · In the first study, researchers manipulated E. coli 's genes to make the bacteria reliably detect high glucose levels in urine--an indicator of diabetes. Once the glucose levels reach a certain ...

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  • Microfluidic smartphone quantitation of Escherichia

    2019-12-1 · For full response curves, a serial dilution of E. coli K12 range from 10 8 to 10 0 CFU/mL in 3% w/w BSA or synthetic urine were loaded into a Nunc maxisorp microplate 400 μL well and 3% w/w BSA used as negative control. The MCF strip was then moved to a new well loaded with 40 μg/mL biotinylated polyclonal detection antibody (detAb) and ...

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  • Rapid test strips detect bacterial contamination in ...

    2012-4-30 · Researchers have developed a rapid testing method using a simple paper strip that can detect E. coli in recreational water within minutes. The new …

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  • E. Coli under the Microscope -Types, Techniques,

    2014-2-26 · Symptomatic E coli UTI: presence of typical lower urinary tract symptoms, pyuria (≥8 leukocytes/mm 3), and E coli ≥10 2 CFU/mL in voided midstream urine; recurrent symptomatic UTI: ≥2 episodes within 12 months: ABO blood group secretor status, UTI history: Daily diaries, clinic visits; saliva, blood and urine samples

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  • Urinary Tract Infections in Older Women

    2012-12-26 · bacteria’s radius or length. In our scheme, the automated detection compares the optical scattering of randomly oriented bacteria versus aligned bacteria. Figure 5 shows the optical scattering for samples of 5·107 colony forming units (CFU)/mL live E. coli in urine and no live E. coli in urine. When the electric field is turned on (between

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  • New Technique for Urine Culture Improves Pathogen ...

    2020-2-1 · E. coli was also the most common bacterium detected by culture (34% of culture positives, 74/217) and Enterococcus faecalis was the second most common (21% of culture positives, 46/217). Download : Download high-res image (452KB) Download : Download full-size image; Figure 1.

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  • Optical detection of asymmetric bacteria utilizing electro ...

    2021-6-25 · E. coli Infection : Escherichia coli 0157:H7 (E. coli 0157:H7) infections are caused by bacterial.E. coli 0157:H7 is one of the most virulent strains and is passed from person to person via contaminated food and water with infected feces. Symptoms include nausea, vomiting, bloody diarrhea, fever, and abdominal pain. Complications can occur in the elderly and in children younger than 6 …

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  • Multiplex PCR Based Urinary Tract Infection (UTI)

    The bacteria used in this work are Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). E. coli is a rod shaped Gram negative bacterium with a length of 2 3 µm [21] and a diameter of 0.5 µm. It is a common cause for bacterial infections in humans and animals [22]. The cytoplasm of E. coli,

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  • E. coli Infection Topic Guide

    2015-5-28 · Engineered bacteria detect cancer and diabetes in urine By Robert F. Service May. 28, 2015 , 4:00 PM Most of us think of bacteria as the enemy, but each of …

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  • Guide to Chronic UTI - Chronic UTI Info

    2019-11-6 · Full-length (V1–V9) amplicons were aligned using MUSCLE 32 and Shannon entropy was calculated at each base position along a single E. coli str. K …

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  • First Instant Test to Detect Presence of E. Coli 1057:H7

    2016-3-8 · Background The objective of our study was the development of a semi-quantitative real-time PCR to detect uropathogens. Two multiplex PCR reactions were designed to detect Escherichia coli, Klebsiella spp., Enterobacter spp., Citrobacter spp., Proteus mirabilis, Enterococcus faecalis, and Pseudomonas aeruginosa. 16S based PCR was performed in parallel to detect Gram-positive and …

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  • Molecular Diagnosis of Urinary Tract Infections by

    2012-4-30 · Researchers have developed a rapid testing method using a simple paper strip that can detect E. coli in recreational water within minutes. The new …

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  • Rapid test strips detect bacterial contamination in ...

    2015-5-27 · Urine Test Could Detect Cancer One Day, As New Method Shows Promise By Jesse Emspak 27 May 2015 Researchers have rewired the genetic circuitry of Escherichia coli bacteria (above) to detect ...

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  • Urine Test Could Detect Cancer One Day, As New

    2014-2-26 · Symptomatic E coli UTI: presence of typical lower urinary tract symptoms, pyuria (≥8 leukocytes/mm 3), and E coli ≥10 2 CFU/mL in voided midstream urine; recurrent symptomatic UTI: ≥2 episodes within 12 months: ABO blood group secretor status, UTI history: Daily diaries, clinic visits; saliva, blood and urine samples

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  • Urinary Tract Infections in Older Women

    2021-1-29 · E.coli (Escherichia coli), is a type of bacteria that normally lives in your intestines.It’s also found in the gut of some animals. Most types of E.coli are harmless and even help keep your digestive tract healthy.But some strains can cause diarrhea if you eat contaminated food or drink fouled water.

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  • Detection Methods for Lipopolysaccharides: Past and ...

    2015-6-5 · Using bacteria to detect cancer in urine. June 5, 2015, 6:00 p.m. SHUTTERSTOCK. Few of us are ever eager to pee into a cup, but there may soon be good reason to be: Researchers at the ...

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  • E. Coli Diagnosis, Procedure and Interpretation | Lab ...

    2018-3-1 · Vial Microbe Media Antibiotic 1 E. coli (ATCC 25922) Luria Broth Control 2 E. coli (ATCC 25922) Luria Broth Imipenem 0.03mg/ml 3 E. coli (ATCC 25922) Luria Broth Imipenem 0.06mg/ml 4 E. coli (ATCC 25922) Luria Broth Imipenem 0.125mg/ml 5 E. coli (ATCC 25922) Luria Broth Imipenem 0.25mg/ml 6 E. coli (ATCC 25922) Luria Broth Imipenem 0.50mg/ml Escherichia coli ATCC 25922 Vs. …

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  • Using bacteria to detect cancer in urine - The Boston

    2019-11-6 · Full-length (V1–V9) amplicons were aligned using MUSCLE 32 and Shannon entropy was calculated at each base position along a single E. coli str. K …

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  • E. coli and Urinary Tract Infections (UTIs) | Everyday

    2018-12-18 · E. coli in the urinary tract causes more than 85 percent of all urinary tract infections, or UTIs. The bacteria, present in fecal matter, typically enters through the urethra and can be especially ...

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  • Questions and Answers | E. coli | CDC

    Escherichia coli (E. coli) bacteria normally live in the intestines of people and animals. Most E. coli are harmless and actually are an important part of a healthy human intestinal tract. However, some E. coli are pathogenic, meaning they can cause illness, either diarrhea or illness outside of the intestinal tract. The types of E. coli that can cause diarrhea can be transmitted through ...

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  • E. coli - Symptoms and causes - Mayo Clinic

    2020-10-10 · Causes. Only a few strains of E. coli trigger diarrhea. The E. coli O157:H7 strain belongs to a group of E. coli that produces a powerful toxin that damages the lining of the small intestine. This can cause bloody diarrhea. You develop an E. coli infection when you ingest this strain of bacteria.. Unlike many other disease-causing bacteria, E. coli can cause an infection even if you ingest ...

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  • Urinary tract infection (UTI) - Diagnosis and treatment ...

    2018-3-12 · E. coli is a member of the bacterial family of Enterobacteriaceae [].It is a most common intestinal microorganism of humans and warm-blooded animals [].Some strains of this bacterium have the pathogenic potential and can cause a wide range of infections from mild to life-threatening conditions, such as traveler’s diarrhea, infant diarrhea, hemorrhagic colitis, hemolytic uremic syndrome ...

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  • Voltammetric determination of the Escherichia coli

    2021-6-24 · Your doctor tells you they want to do a urine culture.It’s a test to check for germs or bacteria in your pee that can cause a urinary tract infection ().. Your urinary tract includes the kidneys ...

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  • Urine Culture Test: Purpose, Duration, Levels of

    2017-3-21 · producing E.coli (VTEC). E.coli O157:H7 The first confir med isolation of E.coli O157:H7 in the United States was in 1975 from a California woman with bloody diarrhoea.The first reported isolation of E.coli O157:H7 from cattle was from a less than 3 week old calf with colibacillosis in Argentina in 1977. The bacterium was first identified E ...

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  • E.coli O157:H7 - Veterinary World

    2015-9-19 · E.coli ATCC 25922 and K.pneumoniae 700603 were used. RESULTS AND DISCUSSION A total of 117 ESBL positive strains isolated from urine specimens were included in this study. 106 strains were E.coli and 11 were K. pneumoniae. Ten (9.4%) E.coli isolates were Vitek (-) and double disk synergy (+) for ESBL detection.

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  • Comparison of conventional method and automatized

    2020-6-16 · The Escherichia coli expression system is one of the most well-characterized classical expression systems for recombinant protein expression in biological science.E. coli offers clear advantages over other expression systems, including a clear genetic background, fast breeding, low cost, high cell density cultures, and high protein expression levels [1,2,3].

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